HIV 1/2 antibody elisa test kitt,96wells/kit, high accuracy Elisa Sandwich method, for quantitative measurement
Materials provided with the kit:
| |
96T |
| Coated Microtiter Plate |
1 bag |
12*8 |
| Conjugate |
1vial |
10.5mL |
| Biotin Working Liquid |
1vial |
3mL |
| Wash Buffer Concentrate (40*) |
1vial |
20mL |
| Substrate A |
1vial |
7mL |
| Substrate B |
1vial |
7mL |
| Stop Solution |
1vial |
6mL |
| Negative Control |
1vial |
1mL |
| Positive Control |
1vial |
1mL |
| Closure Plate Membrane |
3sheet |
|
Note: different batches of reagent kit, and different component can not be exchanged for use. Once open, stable for 3 months at 2-8°C.
INTENDED USE
The Anti-HIV 1+2 ELISA is a qualitative enzyme immunoassay for the in vitro detection of Human
Immunodeficiency Virus (HIV) infection, based on the detection of antibodies to HIV-1 and HIV-2 in human serum or plasma. It is intended for screening of blood donors and diagnosing patients related to infection with HIV.
ASSAY PROCEDURE
1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer with 19 volumes of distilled water,
mix well.
2. Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 50ul of Sample Diluent , dispense 50 ul of sample or negative control or positive control to the respective wells. Gently vibrating the plate.
3. Incubate: Cover the Microplate with plate cover and incubate the Coated Microplate in a thermostat-controlled water-bath or microplate incubator at 37 for 60 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted
washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Conjugate: Add 100ul of conjugate to each well (except the blank well).
6. Incubate: Cover the Microplate and incubate the plate at 37 for 30 minutes.
7. Wash the Plate: Repeat the wash procedure as in step 4.
8. Add Substrate: Add 50ul of Substrate Solution A and 50 ul of Substrate Solution B to each well, mix well.
9. Stop reaction: Add 50ul Stop Solution to each well, mix well.
10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should
| Name |
HIV 1,2 ELISA Test |
| Full name |
Human Immunodeficiency Viruses Type 1,2 Antibody (HIV1,2) ELISA Test Kit |
| Category Name |
HIV ELISA kit |
| Test |
96 Tests |
| Method |
ELISA: Enzyme Linked Immunosorbent Assay |
| Principle |
ELISA: Sandwich; Double antigen |
| Detection Range |
Qualitative: Positive, negative and cutoff calibrators |
| Sample |
100 uL Serum or plasma |
| Specificity |
99.89% |
| Sensitivity |
99.95% |
| Total Time |
15 Min |
| Shelf Life |
12 Months from the manufacturing date |
| ORIENT NEW LIFE MEDICAL CO., LTD. |
| Contact: |
Jerry Meng |
| Email: |
Jerry @ newlifebiotest .com |
| Tel. |
+86 18657312116 |
| SKYPE |
enetjerry
|
Product Tags:
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96 Wells HIV Antibody Test Kit Biotin Working Liquid Quantitative Measurement Images
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